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KMID : 0613820050150040626
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Journal of Life Science
2005 Volume.15 No. 4 p.626 ~ p.632
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Cytoprotective Effects of Polyamines Against Oxidative Stress
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Ahn Seo-Ni
Lee Ji-Young
Chung Hae-Young
Yoo Mi-Ae
Kim Jong-Min
Kim Byeong-Gee
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Abstract
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The polyamines are essential components of all eukaryotic cells and absolutely necessary for cell growth. In the present study, the cytoprotective role of polyamine was characterized. When Ac₂F rat liver cells were treated with 1 mM 2,2’-azobis (2-amidinopropane) dehydrochloride (AAPH), a water soluble free radical initiator, viability of the cells was noticeably decreased due to the increase of reactive oxygen species (ROS). The cytotoxic effect of AAPH as well as ROS generation were significantly inhibited by the treatment of polyamines. Among polyamines, especially spermine at 20 μM concentration exerted over 45% inhibition of AAPH-induced ROS generation. Western blotting was performed to determine whether superoxide dismutase (SOD) or catalase (CAT) expression was involved in oxidative stress. The AAPH treatment blocked both SOD and CAT protein expressions. Spermine could recover those protein expressions to the untreated control levels. According to the result of cyclin E measurement, AAPH might block the entry of the cells into S phase of the cell cycle. The reduced expression of cyclin E protein could be fully recovered by the addition of spermine. The antioxidative effects of spermine was also further proved by the apopotitic morphological analysis using ethidium bromide and acridine orange.
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